The fresco paintings are celebrated for their rich and sometimes rare iconographic details. In the naos, the program includes traditional cycles such as the Great Feasts and the Passion of Christ, as well as unique "fresco-icons" painted directly onto the southern wall, including the Virgin Gorgoepikoos and St. George Diasoritis. The ground floor of the narthex focuses on the Life of the Virgin, the church's patroness, while the upper floor contains cycles dedicated to St. Nicholas and St. Paraskeva. Stylistically, the lead master blended the slim, elegant forms of the Palaeologan Renaissance with dramatic 13th-century inspirations, particularly evident in the emotional intensity of the Lamentation scene.

Among the most historically significant frescoes are the ktitor (founder) portraits located on the upper floor of the narthex. These compositions depict the nobleman founder alongside his wife, children, and a brother, as well as the figures of despot Michael and his wife Helena. These portraits provide an invaluable record of medieval fashion, jewelry, and social hierarchy, offering a precise look at the attire worn by the 14th-century nobility. While many of the original inscriptions have been lost, the surviving imagery continues to offer deep insight into the artistic and religious life of the era.